CHROMATOGRAPHIC SEPARATION OF PHOSVITIN, ALPHA- AND BETA-LIPOVITELLIN OF EGG YOLK GRANULES ON TEAE-CELLULOSE

The two components of lipovitellin and the three major components of yolk granules, phosvitin, alpha and beta-lipovitellin, were separated by gradient elution chromatography on TEAE-cellulose. A 0.2 M phosphate buffer (pH 6.8) had the necessary ionic strength to dissolve these proteins and when applied in this solvent all components except beta-lipovitellin were retained by the column. A linear gradient of ionic strength (limit buffer 0.2 M phosphate plus 0.5 M NaCl) was used to remove the other components. Recovery was essentially complete and the composition and properties of the individual components were similar to those obtained by previous chromatographic methods that gave only partial recovery.