Universal Virus Adsorption on Inert Particles Utilizing the Coated Latex Adsorption Method (CLAM).

Development and instrumentation of a rapid method of virus and viral antibody detection and identification utilizing the Coated Latex Adsorption Method (CLAM) is presented. Comprised of an inert latex particle 'carrier', adsorbing antibody, and detected virus or antibody, the classic CLAM particle may now be classified into two major types (in vitro and in vivo). The in vitro CLAM particle was developed because of the ability of monodispersed latex particles to adsorb, in vitro, a variety of substances and to produce a complex which could be quickly detected within one minute using present day instrument technology. The in vivo CLAM particle retains the basic descriptive properties of the in vitro particle except that it is applied directly to a living host system by various routes of innoculation. By locating at sites of antibody production, the particle acts as tracer, locator, and quantitator of a variety of dynamic biological processes with the host and can thus permit, for example, determination of the earliest possible time of antibody detection after infection.
See also AD-767 041.