Improving enzymatic conversion of lignocellulose to platform sugars

Increasing demand and uncertain availability of fossil fuels urge us to find alternative resources available in large quantities especially for the petrol-based transportation sector. Lignocellulosic biomass, available worldwide in plant cell walls, is a promising alternative feedstock. It can be depolymerised to sugar monomers, which provide potential raw material for sugar platform-based production of fuels and chemicals. However, the enzymatic saccharification of lignocellulose to platform sugars is hindered primarily by the complexity of lignocellulosic substrates as well as by the performance of the hydrolytic enzymes involved. This study focuses on various rate limiting factors such as the decrease in the reactivity and accessibility of the substrates which slow down the hydrolysis, on auxiliary enzymes needed for the efficient solubilisation of cellulose, as well as on the adsorption of enzymes. Consequently, solutions to these limitations were sought to improve the efficiency of biomass conversion processes. Following the morphological and structural changes in the substrate during hydrolysis revealed that the average crystal size and crystallinity of cellulose remained constant while particle size generally decreased (Paper I). In particular, cellulose microfibrils were proposed to be hydrolysed one-by-one in fibre aggregates by peeling off cellulose chains layer-by-layer from the outer crystals of microfibril aggregates. Microscopic observation showed that almost intact particles remained in the residue even after 60% conversion. Lignocellulose is a complex network of lignin and polysaccharides. Lignin was found to impede the hydrolysis of cellulose, and its extensive removal doubled the conversion yields of softwood (Paper II). On the other hand, accumulation of lignin during hydrolysis did not affect hydrolysability by commercial cellulase preparations. Residual hemicelluloses, especially glucomannan, were resistant to enzymatic hydrolysis but could be r
Increasing demand and uncertain availability of fossil fuels urge us to find alternative resources available in large quantities especially for the petrol-based transportation sector. At present, first generation bioethanol and biodiesel are produced worldwide from cornstarch, sugarcane and rapeseed oil. However, fuels produced from these raw materials are not considered sustainable. Thus, recent efforts have been directed towards the use of sustainable raw materials, such as residues from forestry and agriculture as well as municipal wastes. Lignocellulosic biomass, available worldwide in plant cell walls, is a promising alternative feedstock for the production of second generation biofuels. However, the enzymatic saccharification of lignocellulose to platform sugars is hindered primarily by the complexity of lignocellulosic substrates as well as by the performance of the hydrolytic enzymes involved. Therefore, this work focuses on various rate limiting factors such as the decrease in the reactivity and accessibility of the substrates which slow down the hydrolysis, on auxiliary enzymes needed for the efficient solubilisation of cellulose, as well as on the adsorption of enzymes. Consequently, solutions to these limitations were sought to improve the efficiency of biomass conversion processes.