Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients in Iran

We extracted Fusarium solani antigens to evaluate specifi c anti-F. solani IgE in fifty-one patients with asthma (33 men and 18 women) and in 22 non-atopic healthy subjects (15 men and 7 women). F. solani strains were cultured in Sabouraud glucose agar and subjected to cell disruption using the freeze-and-thaw method. The obtained cytoplasmic extracts were analysed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Sensitisation to F. solani antigens has been evaluated in asthmatic patients using the immunoblotting assay. The SDS-PAGE identifi ed 29 protein bands in the cytoplasmic extracts of F. solani isolates, with molecular weights ranging from 24 kDa to 112 kDa. Immunoblotting detected specific anti-F. solani IgE antibody in all asthma patients, but not in the control group. The predominant reactive allergens in patients corresponded to the bands with molecular weights of 24 kDa, 58.5 kDa, 64.5 kDa, 69 kDa, 72 kDa, and 97 kDa. Our results suggest that various allergenic components of F. solani may produce symptoms of asthma in susceptible individuals and they call for further research.
Ekstrahirali smo antigene plijesni Fusarium solani kako bismo izmjerili imunosni odgovor, tj. razine anti-F. solani IgE u 52 bolesnika s astmom (33 muškarca i 18 žena) te u 22 zdrava ispitanika bez atopija (15 muškaraca i sedam žena). Sojevi F. solani uzgojeni su na podlozi glukoznoga Sabouraudova agara i podvrgnuti razbijanju stanica s pomoću metode smrzavanja i odmrzavanja. Dobiveni citoplazmatski ekstrakti analizirani su s pomoću elektroforeze u poliakrilamidnom gelu u prisutnosti natrijeva dodecilsulfata (engl. sodium dodecyl sulphate-polyacrylamide gel electrophoresis, krat. SDS-PAGE). Senzibilizacija na antigene F. solani u bolesnika s astmom utvrđena je s pomoću imunoblot-testa. S pomoću SDS-PAGE-a u citoplazmatskim ekstraktima izolata F. solani dokazano je 29 proteinskih vrpca. Molekulske mase razdvojenih proteina kretale su se u rasponu od 24 kDa do 112 kDa. Imunoblot-test otkrio je specifi čno anti-F. solani IgE- protutijelo u svih bolesnika s astmom, ali niti u jednog ispitanika iz kontrolne skupine. Najčešći su alergeni u bolesnika s astmom odgovarali proteinima ovih molekulskih masa: 24 kDa, 58,5 kDa, 64,5 kDa, 69 kDa, 72 kDa i 97 kDa. Naši nalazi upućuju na to da simptome astme u osjetljivih pojedinaca može izazvati više alergena soja F. solani te da ih treba podrobnije istražiti.