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Single-step antigen loading and activation of dendritic cells by mRNA electroporation for the purpose of therapeutic vaccination in melanoma patients.

Authors :
Bonehill, Aude
Van Nuffel, An M T
Corthals, Jurgen
Tuyaerts, Sandra
Heirman, Carlo
Francois, Violaine
Colau, Didier
van der Bruggen, P
Neyns, Bart
Thielemans, Kris M.
Bonehill, Aude
Van Nuffel, An M T
Corthals, Jurgen
Tuyaerts, Sandra
Heirman, Carlo
Francois, Violaine
Colau, Didier
van der Bruggen, P
Neyns, Bart
Thielemans, Kris M.
Source :
Clinical cancer research, 15 (10
Publication Year :
2009

Abstract

A critical factor determining the effectiveness of currently used dendritic cell (DC)-based vaccines is the DC activation or maturation status. We have recently shown that the T-cell stimulatory capacity of DCs pulsed with tumor-antigen-derived peptides can be considerably increased by activating the DCs through electroporation with mRNA encoding CD40 ligand, CD70, and a constitutively active Toll-like receptor 4 (TriMix DCs). Here, we investigate whether TriMix DCs can be coelectroporated with whole tumor-antigen-encoding mRNA.<br />Journal Article<br />Research Support, Non-U.S. Gov't<br />info:eu-repo/semantics/published

Details

Database :
OAIster
Journal :
Clinical cancer research, 15 (10
Notes :
1 full-text file(s): application/pdf, English
Publication Type :
Electronic Resource
Accession number :
edsoai.ocn803509242
Document Type :
Electronic Resource