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Serotonin 5-HT(2B) receptor loss of function mutation in a patient with fenfluramine-associated primary pulmonary hypertension.

Authors :
Blanpain, Cédric
Le Poul, Emmanuel
Parma, Jasmine
Knoop, Christiane
Detheux, Michel
Parmentier, Marc
Vassart, Gilbert
Abramowicz, Marc
Blanpain, Cédric
Le Poul, Emmanuel
Parma, Jasmine
Knoop, Christiane
Detheux, Michel
Parmentier, Marc
Vassart, Gilbert
Abramowicz, Marc
Source :
Cardiovascular Research, 60 (3
Publication Year :
2003

Abstract

OBJECTIVE: Appetite-suppressant drug fenfluramine is implicated in primary pulmonary hypertension (PPH) but the molecular pathways that mediate this effect are unknown. A mouse model incriminates the serotonin 5-HT(2B) receptor but contrasts with other models where this receptor has been shown to mediate pulmonary arterial relaxation via nitric oxide production. METHODS: We analyzed the human 5-HT(2B) gene in 10 patients with appetite-suppressant drug-associated PPH. RESULTS: A mutation causing premature truncation of the protein product was found in one patient. The mutation was not found in 80 control subjects and no 5-HT(2B) mutation was found in 18 PPH patients not associated with appetite-suppressants. Functional analysis of the transfected receptor expressed either transiently in COS cells or stably in CHO cells demonstrated that the mutated receptor fails to activate the second messenger inositol-phosphates cascade and subsequent intracellular calcium release, in spite of normal expression at the cell membrane. The mutated receptor had no constitutive activity, and produced no dominant negative effect on the wild-type receptor. CONCLUSION: Loss of serotonin 5-HT(2B) receptor function may predispose to fenfluramine-associated PPH in man.<br />Journal Article<br />Research Support, Non-U.S. Gov't<br />info:eu-repo/semantics/published

Details

Database :
OAIster
Journal :
Cardiovascular Research, 60 (3
Notes :
No full-text files, English
Publication Type :
Electronic Resource
Accession number :
edsoai.ocn764587207
Document Type :
Electronic Resource