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Characterization of photosystem 1 chlorophyll a/b-binding apoprotein accumulation in developing soybean using type-specific antibodies
- Source :
- Plant physiology and biochemistry : PPB / Societe francaise de physiologie vegetale. 30(3)
- Publication Year :
- 1992
- Publisher :
- United States: NASA Center for Aerospace Information (CASI), 1992.
-
Abstract
- The structure and supramolecular assembly of the soybean photosystem 1 (PS 1) chlorophyll a/b-binding antenna (LHC 1) was examined. We identified the subunit composition of LHC 1 in soybean and followed the accumulation of individual subunits during light-induced assembly. We observed four LHC 1 subunits, at 23, 22, 21 and 20.5 kDa, obtained partial sequence information by amino-terminal sequence analysis, and classified the 20.5, 22, and 21 kDa subunits as being encoded by type I, II, and IV chlorophyll a/b binding protein genes, respectively. Antisera against LHC 1 subunits were used to follow the accumulation of individual subunits during the light-initiated transition from etioplast to chloroplast. Several points are noteworthy. First, monospecific antibody against the 22 kDa subunit decorated a 25 kDa peptide in etiolated tissue, which declined during maturation. This decline correlated with the light-induced appearance of mature 22 kDa peptide, suggesting a precursor/product relationship. Second, the same antibody identified a 22 kDa protein in mature corn, but not a larger band in etiolated corn, suggesting that LHC 1 accumulation is regulated differently between species before the onset of chlorophyll biosynthesis. Third, the mature 22 kDa subunit appeared somewhat later than the other LHC 1 peptides during greening, implying that this subunit is less intimately associated with the PS1 core than are the subunits appearing earlier in development.
- Subjects :
- Life Sciences (General)
Subjects
Details
- Language :
- English
- ISSN :
- 09819428
- Volume :
- 30
- Issue :
- 3
- Database :
- NASA Technical Reports
- Journal :
- Plant physiology and biochemistry : PPB / Societe francaise de physiologie vegetale
- Notes :
- NAGW-1197, , NAGW-2328
- Publication Type :
- Report
- Accession number :
- edsnas.20040090218
- Document Type :
- Report