A real-time PCR assay for the detection of Campylobacter jejuni in foods after enrichment culture

Research describes the development of a real-time polymerase chain reaction assay based on 5' nuclease system to detect specific C sequence in Campylobacter jejuni. The assay shows linearity of quantification of food contamination over six orders of magnitude, detecting up to 12 genome equivalents. The new assay takes 3 h and applicable to a diverse range of Campylobacter species and other related and unrelated genera.