Synthesis of GDP-mannose and mannosylglycerate from labeled mannose by genetically engineered Escherichia coli without loss of specific isotopic enrichment

Research describes the construction of an Escherichia coli mutant that cannot metabolize mannose but can takeup labeled mannose, mediated by plasmid-encoded enzyme, and preferentially convert to GDP-mannoseand 2-O-alpha-D-mannosyl-D-glycerate preserving specific isotopic enrichment.