[[alpha].sub.1C] ([Ca.sub.v]1.2) L-type calcium channel mediates mechanosensitive calcium regulation

Smooth muscle exhibits mechanosensitivity independent of neural input, suggesting that mechanosensitive pathways reside within smooth muscle cells. The native L-type calcium current recorded from human intestinal smooth muscle is modulated by stretch. To define mechanosensitive mechanisms involved in the regulation of smooth muscle calcium entry, we cloned the [[alpha].sub.1C] L-type calcium channel subunit ([Ca.sub.v]1.2) from human intestinal smooth muscle and expressed the channel in a heterologous system. This channel subunit retained mechanosensitivity when expressed alone or coexpressed with a [[beta].sub.2] calcium channel subunit in HEK-293 or Chinese hamster ovary cells. The heterologously expressed human cardiac [[alpha].sub.1C] splice form also demonstrated mechanosensitivity. Inhibition of kinase signaling did not affect mechanosensitivity of the native channel. Truncation of the [[alpha].sub.1C] COOH terminus, which contains an inhibitory domain and a proline-rich domain thought to mediate mechanosensitive signaling from integrins, did not disrupt mechanosensitivity of the expressed channel. These data demonstrate mechanical regulation of calcium entry through molecularly identified L-type calcium channels in mammalian cells and suggest that the mechanosensitivity resides within the pore forming [[alpha].sub.1C]-subunit. smooth muscle; mechanogated; voltage gated