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Identification, detection, and enumeration of human Bifidobacterium species by PCR targeting the transaldolase gene

Authors :
Requena, Teresa
Burton, Feremy
Matsuki, Takahiro
Munro, Karen
Simon, Mary Alice
Tanaka, Ryuichiro
Watanabe, Koichi
Tannock, Gerald W.
Source :
Applied and Environmental Microbiology. May, 2002, Vol. 68 Issue 5, p2420, 8 p.
Publication Year :
2002

Abstract

Researchers describe a PCR technique based on the transaldolase gene in Bifidobacterium coupled with denaturing gradient gel electrophoresis that can distinguish between species. This is possible because bifidobacteria produce 14 or more different types of transaldolase. Because they produce different electrophoretic patterns, the transaldolase genes must have variable sequences.

Details

ISSN :
00992240
Volume :
68
Issue :
5
Database :
Gale General OneFile
Journal :
Applied and Environmental Microbiology
Publication Type :
Academic Journal
Accession number :
edsgcl.90724031