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Ecdysone-induced expression of the caspase DRONC during hormone-dependent programmed cell death in Drosophila is regulated by Broad-Complex
- Source :
- The Journal of Cell Biology. June 10, 2002, Vol. 157 Issue 6, p985, 11 p.
- Publication Year :
- 2002
-
Abstract
- The steroid hormone ecdysone regulates both cell differentiation and cell death during insect metamorphosis, by hierarchical transcriptional regulation of a number of genes, including the Broad-Complex (BR-C), the zinc finger family of transcription factors. These genes in turn regulate the transcription of a number of downstream genes. DRONC, a key apical caspase in Drosophila, is the only known caspase that is transcriptionally regulated by ecdysone during development. We demonstrate that dronc gene expression is ablated or reduced in BR-C mutant flies. Using RNA interference in an ecdysone-responsive Drosophila cell line, we show that DRONC is essential for ecdysone-mediated cell death, and that dronc upregulation in these cells is controlled by BR-C. Finally, we show that the dronc promoter has BR-C interaction sites, and that it can be transactivated by a specific isoform of BR-C. These results indicate that BR-C plays a key role in ecdysone-mediated caspase regulation.
Details
- ISSN :
- 00219525
- Volume :
- 157
- Issue :
- 6
- Database :
- Gale General OneFile
- Journal :
- The Journal of Cell Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.88999819