Nucleotide binding and nucleotide hydrolysis properties of the ABC transporter MRP6 (ABCC6)

Photolabeling studies show that recombinant rat Mrp6 ABC transporter protein, expressed in Pichia pastoris, is stable and binds and hydrolyzes ATP. The differences in the properties between rMrp6 and the ABC transporter multidrug resistance associated protein 1 (MRP1) can be employed to understand the functional aspects of mutation in pseudoxanthoma elasticum patients.