Comparative genome sequencing for discovery of novel polymorphisms in Bacillus anthracis. (Reports)

Comparison of the whole-genome sequence of Bacillus anthracis isolated from a victim of a recent bioterrorist anthrax attack with a reference reveals 60 new markers that include single nucleotide polymorphisms (SNPs), inserted or deleted sequences, and tandem repeats. Genome comparison detected four high-quality SNPs between the two sequenced B. anthracis chromosomes and seven differences among different preparations of the reference genome. These markers have been tested on a collection of anthrax isolates and were found to divide these samples into distinct families. These results demonstrate that genome-based analysis of microbial pathogens will provide a powerful new tool for investigation of infectious disease outbreaks.
On 4 October 2001, the Centers for Disease Control reported a highly unusual case of inhalational anthrax in a photo editor at a West Palm Beach, Florida, media organization (1). [...]