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A mutational analysis of the PD...D/EXK motif suggests that McrC harbors the catalytic center for DNA cleavage by the GTP-dependent restriction enzyme McrBC from Escherichia coli

Authors :
Pieper, Uwe
Pingoud, Alfred
Source :
Biochemistry. April 23, 2002, Vol. 41 Issue 16, p5236, 9 p.
Publication Year :
2002

Abstract

The mutational analysis of the presumptive nucleolytic center of McrC subunit of the McrBC system of Escherichia coli suggests that McrC mediates DNA cleavage by McrBC. The catalytic center of the McrC is represented by the sequence TD(sup)224 ....D(sup)257 AK.

Details

ISSN :
00062960
Volume :
41
Issue :
16
Database :
Gale General OneFile
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
edsgcl.86433500