Insertion of a reversible redox switch into a rare-cutting DNA endonuclease

Research demonstrates that the DNA cleavage activity of the yeast homing endonuclease can be turned on and off with a redox switch. Data show that Cys-67/Cys-365 variants of the endonuclease exhibit different DNA binding conformations under redox conditions.The DNA binding defects reduce DNA cleavage activities of the disulfide containing proteins.