A novel engineered subtilisin BPN' lacking a low-barrier hydrogen bond in the catalytic triad

Authors :: Stratton, Jennifer R.
Pelton, Jeffrey G.
Kirsch, Jack F.
Source :: Biochemistry. Sept 4, 2001, Vol. 40 Issue 35, p10411, 6 p.
Publication Year :: 2001
Abstract: The hydrolytic catalysis of the mutant subtilisin, Bacillus protease N', with a low-barrier hydrogen bond between the aspartate and histidine residues, is about 8-fold reduced than that of wild-type enzyme. Data indicate that the hydrogen bond is not required for enhanced activity of subtilisin.

Subjects :: Protein engineering -- Research
Hydrogen bonding -- Physiological aspects
Enzyme kinetics -- Analysis
Structure-activity relationships (Biochemistry) -- Analysis
Biological sciences
Chemistry

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