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lacZ fusions report gene expression, don't they?

Authors :
Pessi, Gabriella
Blumer, Caroline
Haas, Dieter
Source :
Microbiology. August, 2001, Vol. 147 Issue 8, p1993, 3 p.
Publication Year :
2001

Abstract

This article examines the conventional molecular biological tool, the lacZ fusions, geneticists use to study regulation of gene expression. Authors provide some cautionary notes to be aware of in such investigations, that reliable gene fusion data are obtained only when transcription start site is precisely determined.

Subjects

Subjects :
Genetic transcription -- Analysis
Genetic translation -- Analysis
Gene fusion -- Analysis
Escherichia coli -- Genetic aspects
Biological sciences

Details

ISSN :
13500872
Volume :
147
Issue :
8
Database :
Gale General OneFile
Journal :
Microbiology
Publication Type :
Academic Journal
Accession number :
edsgcl.78225507

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Authors Abstract Subjects Details