Chemical rescue of phosphoryl transfer in a cavity mutant: a cautionary tale for site-directed mutagenesis

Authors :: Admiraal, Suzanne J.
Meyer, Philippe
Schneider, Benoit
Deville-Bonne, Dominique
Janin, Joel
Herschlag, Daniel
Source :: Biochemistry. Jan 16, 2001, Vol. 40 Issue 2, p403, 11 p.
Publication Year :: 2001
Abstract: Research has been conducted on the nucleoside diphosphate kinase mutant. The ability of this mutant to replace nucleophilic histidine by glycine and to transfer phosphate from ATP to alcohols has been investigated.

Subjects :: Biochemistry -- Research
Phosphorylation -- Physiological aspects
Mutagenesis -- Physiological aspects
Histidine -- Physiological aspects
Glycine -- Physiological aspects
Nucleosides -- Physiological aspects
Alcohols -- Physiological aspects
Biological sciences
Chemistry

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