Analysis of MDR1 P-glycoprotein conformational changes in permeabilized cells using differential immunoreactivity

Authors :: Druley, Todd E.
Stein, Wilfred D.
Roninson, Igor B.
Source :: Biochemistry. April 10, 2001, Vol. 40 Issue 14, p4312, 11 p.
Publication Year :: 2001
Abstract: Results reveal that the conformational changes in the P-glycoprotein are caused by binding and debinding of nucleotides as shown by the use of the conformation-sensitive monoclonal antibody UIC2. The data are interpreted using a conventional E1/E2 model.

Subjects :: Membrane proteins -- Physiological aspects
Conformational analysis -- Research
Biological transport -- Physiological aspects
Glycoproteins -- Physiological aspects
Biological sciences
Chemistry

Tools