Bienzymatic Spectrophotometric Method for Uric Acid Estimation in Human Serum and Urine

In this study, a novel and efficient bienzymatic method for the quantification of uric acid in serum and urine samples was developed. This method is based on the bienzymatic reaction of uricase and peroxidase in the presence of substrates pyrocatechol and 3-methyl-2-benzothiazolinone hydrazone to produce a red colored product. Under optimized conditions, a linearity of uric acid assay was obtained in the ranges of 4 to 384 [micro]M and 2 to 256 [micro]M by kinetic method and fixed time method, respectively. The low limit of detection and limit of quantification were found to be 0.5 and 1.6 [micro]M, respectively. The developed assay was used to quantify uric acid in human serum and urine samples. The present method has good recovery range of 98.3-101.8% and accuracy range of 92.0-101.4%. Hence, the proposed method could be successfully adopted for the quantification of uric acid in clinical laboratories.
Author(s): Ravani Ananda Roopa [sup.1], Kempegowda Mantelingu [sup.2], Mridula Guin [sup.3], Sridhar Bilgumba Thimmaiah [sup.4] Author Affiliations: (1) Department of Chemistry, Pooja Bhagavat Memorial Mahajana Education Centre, , 570016, Mysuru, [...]