Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis

Bub3 gene disruption in mice has been studied. The study shows that there is an essential mitotic spindle checkpoint function in early embryogenesis. Bub3-null embryos seem to be normal to day 3.5 postcoitus. In days 4.5-6.4 they accumulate mitotic errors. Null embryos treated with a spindle depolymerizing agent do not arrest in metaphase. Mitotic disorganization is seen.