Autocloning and amplification of LIP2 in Yarrowia lipolytica

Researchers describe an autocloning method that produces multiple copies of the gene for extracellular lipase in Yarrowia lipolytica. These strains could produce 40 times more lipase per liter than strains containing only one copy of the gene. This makes them a promising source of lipase for industrial use, waste treatment, and pancreatic insufficiency treatment.