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A46R and A52R from vaccinia virus are antagonists of host IL-1 and toll-like receptor signaling
- Source :
- Proceedings of the National Academy of Sciences of the United States. August 29, 2000, Vol. 97 Issue 18, 10162
- Publication Year :
- 2000
-
Abstract
- Poxviruses employ many strategies to evade and neutralize the host immune response. In this study, we have identified two vaccinia virus ORFs, termed A46R and A52R, that share amino acid sequence similarity with the Toll/IL-1 receptor (TLR) domain, a motif that defines the IL-1/Toll-like receptor (TLR) superfamily of receptors, which have a key role in innate immunity and inflammation. When expressed in mammalian cells, the protein products of both ORFs were shown to interfere specifically with IL-1 signal transduction. A46R partially inhibited IL-1-mediated activation of the transcription factor NF[Kappa]B, and A52R potently blocked both IL-1- and TLR4-mediated NF[Kappa]B activation. MyD88 is a TlR domain-containing adapter molecule known to have a central role in both IL-1 and TLR4 signaling. A52R mimicked the dominant-negative effect of a truncated version of MyD88 on IL-1, TLR4, and IL-18 signaling but had no effect on MyD88-independent signaling pathways. Therefore, A46R and A52R are likely to represent a mechanism used by vaccinia virus of suppressing TIR domain-dependent intracellular signaling.
- Subjects :
- Immunity -- Research
Vaccinia -- Research
Science and technology
Subjects
Details
- ISSN :
- 00278424
- Volume :
- 97
- Issue :
- 18
- Database :
- Gale General OneFile
- Journal :
- Proceedings of the National Academy of Sciences of the United States
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.66031264