Specificity of lipid incorporation is determined by sequences in the N-terminal 37% of Apo

Specificity of lipid incorporation has been determined by sequences in the N-terminal 37% of apolipoprotein B (ApoB). C127 cells were permanently transfected to secrete the N-terminal 29, 32.5 or 37% of apoB. It had been shown already that mouse mammary carcinoma-derived (C127) cells, transfected to express the N-terminal 17% of human apoB (apoB-17), secreted mostly lipid-poor apoB-17 with a peak density less than 1.21 g/ml. the beta-sheets at and beyond apoB-29 are critical for recruiting triacylglycerol (TAG) into nascent lipoprotein.