Enzyme-induced strain-distortion in the ground-state ES complex in beta-lactamase catalysis revealed by FTIR

Authors :: Hokenson, Mark J.
Cope, Gregory A.
Lewis, Evan R.
Oberg, Keith A.
Fink, Anthony L.
Source :: Biochemistry. May 30, 2000, Vol. 39 Issue 21, p6538, 8 p.
Publication Year :: 2000
Abstract: Enzyme-induced strain-distortion in the ground-state ES complex in beta-lactamase catalysis has been shown by the use of Fourier transform infrared (FTIR) spectroscopy to exist. A major source of resistance to beta lactam antibiotics is beta-lactamases. Enzyme-facilitated hydrolysis caused by substrate distortion on binding to the enzyme is suggested by the very weak catalytic activity of the S70A beta-lactamase.

Subjects :: Enzymes -- Physiological aspects
Beta lactamases -- Physiological aspects
Bacillus (Bacteria) -- Physiological aspects
Penicillin -- Physiological aspects
Catalysis -- Physiological aspects
Fourier transform infrared spectroscopy -- Usage
Drug resistance in microorganisms -- Research
Binding sites (Biochemistry) -- Physiological aspects
Structure-activity relationship (Pharmacology) -- Research
Biological sciences
Chemistry

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