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Role of Lys 100 in human dihydroorotate dehydrogenase: mutagenesis studies and chemiccal rescue by external amines

Authors :
Jiang, Wenjun
Locke, Gregory
Harpel, MarkR.
Copeland, Robert A.
Marcinkeviciene, Jovita
Source :
Biochemistry. July 11, 2000, Vol. 39 Issue 27, p7990, 8 p.
Publication Year :
2000

Abstract

Results reveal that at least one lysyl residue255 is involved in catalysis of the human dihydroorotate dehydrogenase as shown by chemical mutagenesis. In addition, it is seen that substitution of lysyl100 abolishes the activity and is restored by exogenously added primary amines.

Details

ISSN :
00062960
Volume :
39
Issue :
27
Database :
Gale General OneFile
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
edsgcl.63969633