Succinyl coenzyme A synthetase of Pseudomonas aeruginosa with a broad specificity for nucleoside triphosphate (NTP) synthesis modulates specificity for NTP synthesis by the 12-kilodalton form of nucleoside diphosphate kinase

Results reveal that in Pseudomonas aeruginosa both ATP and GTP are synthesized via succinyl coenzyme A synthetase using succinyl-CoA, inorganic phosphate, and an equimolar mixture of ADP and GDP. Data indicate that although the enzyme can synthesize other nucleotide phosphates, it preferentially synthesizes ATP and GTP.