Bifidobacterium animalis Protects Intestine from Damage Induced by Zinc Deficiency in Rats

We investigated the potential beneficial effects of Bifidobacterium animalis on intestinal damage using zinc-deficient (ZD) rats as a model for intestinal alterations. The ZD rats were fed diets containing 1 mg Zn/kg for 20 (ZD(sub 20) or 40 (ZD(sub 40)) d to induce damage that differed in severity. Subgroups of these rats, the ZD(sub 20) + B and ZD(sub 40) + B groups, received a suspension of B. animalis (3.5 x 10(super 8) colony forming units) daily for the last 10 d. Another subgroup, the ZD(sub 40) + B + 7 d group, was fed the ZD diet for 7 d after the B. animalis treatment period. Zinc deficiency induced ulcerations, edema, inflammatory cell infiltration and dilatation of blood vessels in duodenum, jejunum and ileum, with increasing severity between 20 and 40 d of zinc deficiency. The mucosa of the ZD(sub 20) + B group was well preserved, and most of the morphologic alterations induced by zinc deficiency were normalized in the ZD(sub 40) + B group. The high fecal concentrations of B. animalis in the ZD(sub 40) + B and ZD(sub 40) + B + 7 d groups indicate that these bifidobacteria survived passage through the gastrointestinal tract and proliferated. Electron microscopy confirmed the elevated numbers of bifidobacteria in cecum. Treatment with B. animalis resulted in greater epithelial cell proliferation and disaccharidase activities in the ZD(sub 40) + B group compared with the ZD(sub 40) group. These findings indicate that B. animalis can protect the intestine from alterations induced by zinc deficiency, suggesting that this bacterium may play a role in intestinal mucosal defense.