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Colistin-resistant Escherichia coli carrying mcr-1 in food, water, hand rinse, and healthy human gut in Bangladesh
- Source :
- Gut Pathogens. January 27, 2020, Vol. 12 Issue 1
- Publication Year :
- 2020
-
Abstract
- Background One of the most significant public health concerns in today's world is the persistent upsurge of infections caused by multidrug resistant bacteria. As a result, clinicians are being forced to intervene with either less effective backup drugs or ones with substantial side-effects. Colistin is a last resort antimicrobial agent for the treatment of infections caused by multi-drug resistant gram-negative bacteria. Methods Escherichia coli (n = 65) isolated from street food (n = 20), hand rinse (n = 15), surface water (n = 10), and healthy human stool (n = 20) were tested for colistin resistance gene mcr-1 and response to antimicrobial agents. Antimicrobial resistance genes and virulence genes were detected by employing polymerase chain reaction. DNA fingerprinting of the strains were determined by pulsed-field gel electrophoresis. Results Screening of E. coli allowed us to confirm colistin resistance marker gene mcr-1 in 13 strains (street food, n = 4; hand rinse, n = 2; surface water, n = 4; and stool, n = 3); and two of these E. coli strains carrying mcr-1 harbored bla.sub.TEM gene encoding extended spectrum beta lactamase. Antibiotic assay results revealed all 13 E. coli strains carrying mcr-1 to be multi-drug resistant (MDR), including to colistin. The minimum inhibitory concentration (MIC) for colistin ranged from 2 to 6 [mu]g/ml. DNA sequencing confirmed homogeneity of the nucleotide sequence for mcr-1, but the E. coli strains were heterogenous, as confirmed by pulsed-field gel electrophoresis suggesting horizontal transmission of colistin resistance in Bangladesh. Conclusion Widespread dissemination of E. coli strains carrying mcr-1 encoding resistance to colistin in the present study is alarming as this is the last resort drug for the treatment of infections caused by MDR gram-negative bacteria resistant to almost all drugs used commonly. Keywords: Colistin, mcr-1, ESBL, Multi-drug resistant (MDR), Minimum inhibitory concentration (MIC), Horizontal transmission<br />Author(s): Fatema-Tuz Johura[sup.1], Jarin Tasnim[sup.1], Indrajeet Barman[sup.1], Sahitya Ranjan Biswas[sup.1], Fatema Tuz Jubyda[sup.1], Marzia Sultana[sup.1], Christine Marie George[sup.2], Andrew Camilli[sup.3], Kimberley D. Seed[sup.4], Niyaz Ahmed[sup.1] and Munirul Alam[sup.1] Background Antimicrobial [...]
- Subjects :
- Bacteria -- Health aspects
Colistin -- Health aspects
Virulence (Microbiology) -- Health aspects
DNA sequencing -- Health aspects
Water resources -- Bangladesh -- Health aspects
Public health -- Health aspects
Beta lactamases -- Health aspects
Microbial drug resistance -- Care and treatment -- Health aspects
Infection -- Care and treatment -- Health aspects
Methylene blue -- Health aspects
Escherichia coli -- Health aspects
Base sequence
Antibacterial agents
Gram-negative bacteria
DNA
Genes
Electrophoresis
Polymerase chain reaction
Water
Drug resistance
Public health movements
Health
Subjects
Details
- Language :
- English
- ISSN :
- 17574749
- Volume :
- 12
- Issue :
- 1
- Database :
- Gale General OneFile
- Journal :
- Gut Pathogens
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.616347005
- Full Text :
- https://doi.org/10.1186/s13099-020-0345-2