Tracing the origin of adult intestinal stem cells

Adult intestinal stem cells are located at the bottom of crypts of Lieberkühn, where they express markers such as LGR5.sup.1,2 and fuel the constant replenishment of the intestinal epithelium.sup.1. Although fetal LGR5-expressing cells can give rise to adult intestinal stem cells.sup.3,4, it remains unclear whether this population in the patterned epithelium represents unique intestinal stem-cell precursors. Here we show, using unbiased quantitative lineage-tracing approaches, biophysical modelling and intestinal transplantation, that all cells of the mouse intestinal epithelium--irrespective of their location and pattern of LGR5 expression in the fetal gut tube--contribute actively to the adult intestinal stem cell pool. Using 3D imaging, we find that during fetal development the villus undergoes gross remodelling and fission. This brings epithelial cells from the non-proliferative villus into the proliferative intervillus region, which enables them to contribute to the adult stem-cell niche. Our results demonstrate that large-scale remodelling of the intestinal wall and cell-fate specification are closely linked. Moreover, these findings provide a direct link between the observed plasticity and cellular reprogramming of differentiating cells in adult tissues following damage.sup.5-9, revealing that stem-cell identity is an induced rather than a hardwired property. Lineage tracing, biophysical modelling and intestinal transplantation approaches are used to demonstrate that, in the mouse fetal intestinal epithelium, cells are highly plastic with respect to cellular identity and, independent of LGR5 expression and cell position, can contribute to the adult stem cell compartment.
Author(s): Jordi Guiu [sup.1] , Edouard Hannezo [sup.2] [sup.3] , Shiro Yui [sup.1] [sup.13] , Samuel Demharter [sup.1] , Svetlana Ulyanchenko [sup.1] , Martti Maimets [sup.1] , Anne Jørgensen [sup.4] [...]