Catalytic electron transport in Chromatium vinosum [NiFe]-hydrogenase: application of voltammetry in detecting redox-active centers and establishing that hydrogen oxidation is very fast even at potentials close to the reversible H(sup)+/H(sub)2

Results show that Chromatium vinosum hydrogenase, adsorbed at a pyrolytic graphite edgeplane electrode, exhibits high level of hydrogen oxidation activity which is limited by the rate of substrate diffusion as measured by protein film voltammetry.