Phage display of a biologically active Bacillus thuringiensis toxin

Research was conducted to examine the expression of Bacillus thuringiensis (Cryl1Ac) toxin in two different phage display vector systems. Both systems successfully permit the expression of active toxin as a fusion protein on filamentous phage. Results demonstrate that activated Cryl1Ac can be expressed in E. coli as a translational fusion with the minor phage coat protein of filamentous phage. The phage display of B. thuringiensis toxins exhibits many advantages over previous expression systems for the proteins.