Contribution of cytochrome P-450 4A1 and 4A2 to vascular 20-hydroxyeicosatetraenoic acid synthesis in rat kidneys

A study was conducted on antisense oligonucleotides (ODNs) designed after the active cytochrome P4A1 (CYP4A1) and CYP4A2/4A3 expression in Sprague-Dawley rats. The effectiveness of the antisense ODNs in inducing isoform-specific inhibition of the in vitro and in vivo synthesis of the 20-hydroxyeicosatetraenoic acid (20-HETE), the metabolite formed after the omega-hydroxylation of arachidonic acid was also investigated. The findings imply that CYP4A1 holds the potential of regulating renal function and blood pressure based on its 20-HETE synthetisizing activity in the rat kidney.