Optical studies of single molecules at room temperature

New developments in optical studies of single molecules at room temperature have been assembled with concentration on underlying principles and the potential of single-molecule experiments. Single-molecule-studies examples include those that involve photochemistry and photophysics experiments related to spectral fluctuations, single-molecule measurements, Raman spectroscopy, conformational dynamics, diffusional motions, fluorescence resonant energy transfer, enzymatic turnovers and exciton dynamics. These studies are an illustration of the information that can be obtained with the single-molecule approach not easily seen in ensemble-averaged measurements.