The Role of Next-Generation Sequencing in the Cytologic Diagnosis of Pancreatic Lesions

Context.--Integration of the analysis of genetic markers with endoscopic ultrasound-guided fine-needle aspiration and cytologic evaluation has increased the accuracy of the preoperative diagnosis of pancreatic lesions. The application of high-throughput gene panel analysis using next-generation sequencing platforms is now offering a great opportunity for further improvements. Objective.--To review the application of next-generation sequencing to the preoperative diagnosis of pancreatic lesions. Data Sources.--For data acquisition, a PubMed search using the terms next-generation sequencing, pancreas, pancreatic lesions, pancreatic tumors, and EUS-FNA was performed covering the years 2000-2017. Conclusions.--KRAS remains the gene most widely studied for preoperative single-gene tests. Next-generation sequencing reliably allows analysis of multiple gene markers starting from limited amounts of DNA. The study of multigene panels has become a very attractive option for the management and preoperative risk stratification of patients with pancreatic cancer. doi: 10.5858/arpa.2017-0215-RA
Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive types of cancer in humans and is usually diagnosed at an advanced stage. (1) The management of patients with a [...]