Population and individual identification of coho salmon in British Columbia through parentage-based tagging and genetic stock identification: an alternative to coded-wire tags

Parentage-based tagging (PBT) and genetic stock identification (GSI) were used to identify individual coho salmon (Oncorhynchus kisutch) to specific populations and brood years. In total, 20 242 individuals from 117 populations were genotyped at 304 single nucleotide polymorphisms (SNPs) via direct sequencing of amplicons. Coho salmon from 15 populations were assigned via parentage analysis that required the genotypes of both parents. The overall accuracy of assignment for 1939 coho salmon to the correct population was 100%, and to correct brood year within population was also 100%. Inclusion of individuals requiring only a single parental genotype for identification resulted in assignments of 2101 individuals, with an accuracy of 99.95% (2000-2001) to population and 100.0% to age. With 23 regions defined by the coded-wire tag (CWT) program, and individuals displaying an assignment probability Le marquage base sur l'ascendance (PBT) et l'identification genetique des stocks (GSI) ont ete utilises pour relier des saumons cohos (Oncorhynchus kisutch) individuels a des populations et annees d'eclosion precises. Au total, 20 242 individus issus de 117 populations ont ete genotypes sur 304 polymorphismes nucleotidiques (SNP) par sequencage direct d'amplicons. Des saumons cohos issus de 15 populations ont ete affectes par une analyse de l'ascendance qui necessitait les genotypes des deux parents. L'exactitude globale de l'affectation de 1939 saumons cohos a la bonne population etait de 100 %, et a la bonne annee d'eclosion au sein de la population, egalement de 100 %. L'inclusion d'individus necessitant seulement un genotype parental pour l'identification s'est traduite par l'affectation de 2101 individus, avec une exactitude de 99,95 % (2000-2001) a la bonne population, et de 100 % a la bonne classe d'age. En tenant compte des 23 regions definies par le programme de micromarques magnetisees codees (CWT) et en excluant de l'analyse les individus presentant une probabilite d'affectation de
Introduction Coho salmon (Oncorhynchus kisutch) are produced in hatcheries in British Columbia, Canada, with approximately seven million juveniles released annually. In some hatcheries, a portion of the juveniles is marked [...]