Development of a toolkit for early precision immunochromatographic diagnosis

A device is developed for automated immunochromatographic assay, designed for one-time tests at the place of occurrence of a disease. The device comprises a turntable platform for up to five strips placed simultaneously, a unit for sample collection and microdosing to a test strip, a detection unit, and a microprocessor control system. A manual, semiautomatic, or automatic operation mode may be selected. Colloidal quantum dots with a luminescence peak from the visible to near-infrared spectral region (600-800 nm) are used as fluorescent labels. A UV-LED or a laser at 365-480 nm can be used as an excitation system; the detection device is a spectrometer with an operation range of 500-800 nm, based on a CCD series of TCD1304DG sensors (Toshiba). RFID tags of samples can re read and the analysis results can be sent to an external computer. The device is made in a plastic case for carrying. Keywords: lateral flow immunoassay, immunochromatographic assay, reader, test strips, fluorescent label, quantum dots, antibody conjugate
Currently, immunochemical methods based on highly specific interactions of analytes with corresponding antibodies are increasingly widely used in medical and veterinary diagnostics [1]. A version of immunoassay is immunochromatographic assay [...]