Evaluation of 1,10-phenanthroline-5,6-dione as redox mediator for glucose oxidase

A low-cost and simply fabricated amperometric glucose biosensor based on glucose oxidase (GOx) and 1,10-phenanthroline-5,6-dione (PD) modified graphite rod electrode was developed. The electrode exhibited good electrocatalytic activity with a well-defined hyperbolic dependence of amperometric signal upon glucose concentration in the presence and in the absence of oxygen. The electrode showed a good reproducibility and repeatability with relative standard deviation of less than 3 and 5%, respectively. The interaction of GOx with PD mediator was confirmed by means of UV-'Vis absorbance spectroscopy. Keywords: phenanthroline derivative, 1,10-phenanthroline-5,6-dione, glucose oxidase, glucose biosensor, amperometric sensors, electrocatalytic activity DOI: 10.1134/S1061934816010044
The development of biosensors is one of the most important directions in bioanalytical chemistry, because biosensors could be successfully applied for the determination of some toxic compounds [1] and biologically [...]