Determination of bioactive compounds by catalytic method coupled with planar chromatography

The promoting effect of collagen and inhibiting effect of α-alanine on the catalytic activity of copper(II) in the oxidation of 3,3',5,5'-tetramethylbenzidine with ammonium persulfate and the reduction of iron(III) with sodium thiosulfate, respectively, have been found. A method combining planar chromatography with the subsequent catalytic determination of collagen after desorption from a plate and methods of sorption--catalytlic determination of histamine and α-alanine directly on the support have been developed with limits of detection 2 x [10.sup.-14], 5 x [10.sup.-7], and 8 x [10.sup.-14] M for histamine, α-alanine, and collagen, respectively. Separation by paper and thin-layer chromatography allowed a substantial increase in the selectivity of catalytic determination. The methods were used to determine histamine in human saliva and collagen and α-alanine in cosmetics. Keywords: histamine, α-alanine, collagen, paper chromatography, thin-layer chromatography, catalytic determination, human saliva, cosmetics DOI:10.1134/S1061934813060038
INTRODUCTION Catalytic methods of analysis are characterized by high sensitivity of determination of both metal catalysts and organic compounds influencing their catalytic activity [1, 2]. A substantial disadvantage of these [...]