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Indium--tin oxide coated microfabricated device for the injection of a single cell into a fused silica capillary for chemical cytometry
- Source :
- Analytical Chemistry. Dec 1, 2010, Vol. 82 Issue 23, p9959, 3 p.
- Publication Year :
- 2010
-
Abstract
- A microfabricated device is described for the capture and injection of a single mammalian cell into a fused silica capillary for subsequent analysis by chemical cytometry. The device consists of a 500 [micro]m diameter well made from polydimethylsiloxane on an indium--tin oxide coated microscope slide. The bottom of the well contains a 2 [micro]m high aperture, which was designed to block passage of cells. A cellular suspension was allowed to settle on the device, and aspiration through the aperture was used to trap a single NG-108 cell. Untrapped cells were washed from the device, and a 150 [micro]m outer diameter and 50 [micro]m inner diameter capillary was placed in the well. To inject a cell, voltage was applied to the indium--tin oxide while simultaneously applying vacuum at the distal end of the capillary. 10.1021/ac1022716
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 82
- Issue :
- 23
- Database :
- Gale General OneFile
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.245167226