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Monitoring the uptake of glycosphingolipids in Plasmodium falciparum-infected erythrocytes using both fluorescence microscopy and capillary electrophoresis with laser-induced fluorescence detection
- Source :
- Analytical Chemistry. Dec 1, 2010, Vol. 82 Issue 23, p9955, 4 p.
- Publication Year :
- 2010
-
Abstract
- The metabolism of glycosphingolipids by the malaria-causing parasite Plasmodium falciparum plays an important role in the progression of the disease. We report a new and highly sensitive method to monitor the uptake of glycosphingolipids in infected red blood cells (iRBCs). A tetramethylrhodamine-labeled glycosphingolipid (GM1TMR) was used as a substrate. Uptake was demonstrated by fluorescence microscopy. The iRBCs were lysed with a 15% solution of saponin and washed with phosphate buffered saline to release intact parasites. The parasites were further lysed and the resulting homogenates were analyzed by capillary electrophoresis with laser-induced fluorescence detection. The lysate from erythrocytes infected at 1% parasitemia generated a signal 20 standard deviations larger than uninfected erythrocytes, which suggests that relatively low infection levels can be studied with this technique. 10.1021/ac1021776
- Subjects :
- Electrophoresis -- Methods
Glycolipids -- Chemical properties
Glycolipids -- Health aspects
Sphingolipids -- Chemical properties
Sphingolipids -- Health aspects
Fluorescence microscopy -- Methods
Erythrocytes -- Chemical properties
Erythrocytes -- Health aspects
Plasmodium falciparum -- Health aspects
Lasers -- Usage
Laser
Chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 82
- Issue :
- 23
- Database :
- Gale General OneFile
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.245167225