Artificial triple Wolbachia infection in Aedes albopictus yields a new pattern of unidirectional cytoplasmic incompatibility

The generation of a stable triple infection is described by introducing Wolbachia wRi from Drosophila simulans into a naturally superinfected Aedes albopictus strain. The triple-infected strain has exhibited a pattern of unidirectional incompatibility with the naturally infected strain and the unidirectional cytoplasmic incompatibility (CI) has shown that the artificial cytotype has served as an appropriate priate vehicle for gene drive.