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Kinetic analysis of the bisubstrate cysteine desulfurase SufS from Bacillus subtilis

Authors :
Selbach, Bruna
Earles, Emily
Dos Santos, Patricia C.
Source :
Biochemistry. Oct 12, 2010, Vol. 49 Issue 40, 8794-8802
Publication Year :
2010

Abstract

A sensitive method for detection of alanine formed in the SufS reaction was developed to identify the participation of Fe-S cluster scaffold SufU as a substrate in the cysteine desulfurase gene sufS catalytic mechanism in Gram-positive bacteria, such as Bacillus subtilis. Steady-state kinetic analysis of alanine formation provided evidence for the double-displacement mechanism (ping-pong) of the cysteine:SufU sulfurtransferase reaction catalyzed by SufS.

Details

Language :
English
ISSN :
00062960
Volume :
49
Issue :
40
Database :
Gale General OneFile
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
edsgcl.239938218