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Defining a stem length-dependent binding mechanism for the cocaine-binding aptamer. A combined NMR and calorimetry study
- Source :
- Biochemistry. Oct 5, 2010, Vol. 49 Issue 39, 8478-8487
- Publication Year :
- 2010
-
Abstract
- A combined approach of NMR spectroscopy and isothermal titration calorimetry (ITC) is used for determining the ligand-binding mechanism used by a cocaine-binding aptamer. Chemical shift perturbation data and the observation of intermolecular NOEs have shown that the three-way junction is the site of ligand binding.
Details
- Language :
- English
- ISSN :
- 00062960
- Volume :
- 49
- Issue :
- 39
- Database :
- Gale General OneFile
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.239868724