Hydroxyapatite-mediated separation of double-stranded DNA, single-stranded DNA, and RNA genomes from natural viral assemblages

Hydroxyapatite chromatography is used for fractionating double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), dsRNA and ssRNA genomes from known bacteriophages. Taxonomic analysis has shown that the majority of environmental sequences, regardless of their source nucleic acid, are very similar to dsDNA viruses, indicating the bias of viral metagenomic sequence databases.