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Temperature and time-resolved total internal reflectance fluorescence analysis of reusable DNA hydrogel chips
- Source :
- Analytical Chemistry. July 15, 2010, Vol. 82 Issue 14, p6124, 8 p.
- Publication Year :
- 2010
-
Abstract
- Total internal reflection fluorescence (TIRF) coupled with hydrogel--DNA droplet microarrays covalently bound on PMMA substrates presents a reusable, sensitive platform for evaluating DNA hybridization and for rapid biochip development. Hydrogel microarrays, which contain covalently bound DNA probes, are created via a simple printing and photocross-linking process. TIRF measurements of the arrays display robust reusability, show linear sensitivity down to 5 fmol of fluorescently labeled target DNA, and are sensitive to single basepair mismatches. Additionally, the ability to interrogate larger DNA is shown through studies with PCR amplification hybridization. We conclusively demonstrate an efficient, reproducible, low cost platform for DNA hybridization studies that could be used for fast high-throughput diagnostics as well as biochip development. 10.1021/ac1008578
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 82
- Issue :
- 14
- Database :
- Gale General OneFile
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsgcl.232715771