Characterization of chloride-depleted human sulfite oxidase by electron paramagnetic resonance spectroscopy: experimental evidence for the role of anions in product release

Authors :: Rajapakshe, Asha
Emesh, Safia
Astashkin, Andrei V.
Enemark, John H.
Johnson-Winters, Kayunta
Nordstrom, Anna R.
Meyers, Kimberly T.
Source :: Biochemistry. June 29, 2010, Vol. 49 Issue 25, 5154-5159
Publication Year :: 2010
Abstract: The samples of the wild type and various mutants of human sulfite oxidase (SO) that are depleted of chloride are prepared and purified. The studies have shown that the depletion of chloride has no effect on the formation of the high-pH (hpH) species.

Subjects :: Hydrogen-ion concentration -- Research
Gene mutations -- Research
Dichloropropane -- Research
Electron paramagnetic resonance spectroscopy -- Research
Chlorides -- Research
Biological sciences
Chemistry

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