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Sox9 expression marks a subset of CD24-expressing small intestine epithelial stem cells that form organoids in vitro

Authors :
Gracz, Adam D.
Ramalingam, Sendhilnathan
Magness, Scott T.
Source :
The American Journal of Physiology. May, 2010, Vol. 298 Issue 5, pG590, 11 p.
Publication Year :
2010

Abstract

The inability to identify, isolate, and culture intestinal epithelial stem cells (IESCs) has been prohibitive to the study and therapeutic utilization of these cells. Using a [Sox9.sup.EGFP] mouse model, we demonstrate that [Sox9.sup.EGFP] fluorescence signatures can be used to differentiate between and enrich for progenitors ([Sox9.sup.EGFPsubLo]) and multipotent IESCs ([Sox9.sup.EGFPlo]). [Sox9.sup.EGFPlo] cells generate 'organoids' in a recently defined culture system that mimics the native IESC niche. These organoids possess all four differentiated cell types of the small intestine epithelium, demonstrating the multipotent capacity of [Sox9.sup.EGFPlo] cells. Our results are consistent with the previously reported observation that single IESCs generate cryptlike units without a detectable mesenchymal cell component. A prospective search revealed that CD24 is expressed in the [Sox9.sup.EGFPlo] population and marks IESCs that form organoids in culture. CD24 represents the first cell surface marker that facilitates fluorescence-activated cell sorting enrichment of IESCs with widely available antibodies without requiring a specialized fluorescent reporter gene mouse model. SOX factors; intestinal epithelial culture doi: 10.1152/ajpgi.00470.2009.

Details

Language :
English
ISSN :
00029513
Volume :
298
Issue :
5
Database :
Gale General OneFile
Journal :
The American Journal of Physiology
Publication Type :
Academic Journal
Accession number :
edsgcl.227181761