Proton-evolved local-field solid-state NMR studies of cytochrome [b.sub.5] embedded in bicelles, revealing both structural and dynamical information

Two-dimensional proton-evolved local-field (2D PELF) pulse sequences are implemented by using either composite zero cross-polarization (COMPOZER-CP) or windowless isotropic mixing (WIM) for magnetization transfer. The results from magnetically aligned bicelles containing uniformly [super 15]N-labeled cytochrome [b.sub.5] have shown that the PELF-based experimental approaches have a major impact on solid-state NMR spectroscopy of membrane proteins and other membrane-associated molecules in magnetically aligned bicelles.